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Zymo Research
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Quick-RNA Miniprep Kit
• High-quality total RNA (including small RNAs) from a wide range of samples – single
to 106 cells.
• Isolate small and large RNAs into separate fractions (optional).
• DNA-free RNA for use in any downstream application. DNase I included.
• No organic denaturants!
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

HIGHLIGHTS

  • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
  • DNA-Free: Genomic DNA removal column and DNase I included.
  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
DESCRIPTION

The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with Zymo-Spin™ column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes. The procedure is simple. Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns. The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.

Learn More


EquipmentMicrocentrifuge, vortex
PurityRNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample SourceCells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™.
Size RangeTotal RNA ≥ 17 nt
Yield100 µg RNA (binding capacity), ≥50 µl (elution volume)


Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?

Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).

Q2: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Q3: Can samples be stored in RNA Lysis Buffer prior to processing?

Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Q4: Will the kit isolate small RNAs?

Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.

Q5: I ran out of RNA Wash Buffer. Can I use something else?

Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

Q6: Is it possible to extract proteins with the Quick-RNA kit?

Yes, proteins can be acetone precipitated from the column flowthrough. Please request supplementary protocol from Zymo Research Technical Support.

Q7: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q8: How to store DNase-I following resuspension?

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

Q9: Is DNase I available for individual purchase?

Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.



주문정보

CAT.No 품명 규격 비고
R1054T Quick-RNA™ Miniprep Kit 10 preps. Format: Spin-Column Elution Volume: ≥ 30 μl Binding Capacity: 100 μg Sample Size: ≤ 107 cells Processing Time: 10 minutes
R1054 Quick-RNA™ Miniprep Kit 50 preps. Format: Spin-Column Elution Volume: ≥ 30 μl Binding Capacity: 100 μg Sample Size: ≤ 107 cells Processing Time: 10 minutes
R1055 Quick-RNA™ Miniprep Kit 200 preps. Format: Spin-Column Elution Volume: ≥ 30 μl Binding Capacity: 100 μg Sample Size: ≤ 107 cells Processing Time: 10 minutes