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Direct-zol-96 MagBead RNA
현재 Tri-reagent가 포함된 카다로그넘버 제품은 수입이 불가 합니다.

• High-throughput, magnetic bead based purification of high-quality (DNA-free)
total RNA directly from samples stored in TRIzol®, TRI Reagent® and all other acidguanidinium-
phenol based reagents.
• Eliminates phase separation and precipitation procedures.
• Efficient, broad range purification of small and large RNAs from cells, tissues, biological
liquids, in vitro transcripts, etc.
• Automation ready!
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

HIGHLIGHTS

  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.
DESCRIPTION

The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.

Learn More


CompatibilityTRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
EquipmentMicrocentrifuge, vortex, magstand
Sample InactivationTRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample SourceAny sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size RangeTotal RNA ≥ 17 nt
Yield10 µg RNA (binding capacity), ≥ 30 µl (elution volume)


Q1: Is Direct-zol suitable for very small numbers of cells?

Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

Q2: Is DNase I available for individual purchase?

All kit components are available for purchase separately.

Q3: How to store DNase-I following resuspension?

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

Q4: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q5: Is the kit compatible with samples stored in DNA/RNA Shield?

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

Q10: Which phenol-based reagents are compatible with Direct-zol?

The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.



 

주문정보

CAT.No 품명 규격 비고
R2100 Direct-zol™ 96 Magbead RNA Kit 2 x 96 preps. Format: Magnetic Beads Elution Volume: 50 μl Binding Capacity: 10 μg/prep. Size Limits: 17 - 200 nt Processing Time: 45 minutes
R2102 Direct-zol™ 96 Magbead RNA Kit 4 x 96 preps. Format: Magnetic Beads Elution Volume: 50 μl Binding Capacity: 10 μg/prep. Size Limits: 17 - 200 nt Processing Time: 45 minutes
R2104 Direct-zol™ 96 Magbead RNA Kit 8 x 96 preps. Format: Magnetic Beads Elution Volume: 50 μl Binding Capacity: 10 μg/prep. Size Limits: 17 - 200 nt Processing Time: 45 minutes