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Universal cDNA Reverse Transcribed by Random Hexamer: Human Normal Tissues

Species: Human
Applications: NGS, qPCR
BioChain’s Human Universal cDNA is prepared from major organs of both male and female adult donors. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning human genes.

02)576-6303
info@kyongshin.co.kr

제품소개

BioChain's universal cDNA is prepared using the following method. 10 µg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. The RT Reaction was stopped by heating at 65ºC for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is about 2.5 ng/ul. 1 µ l cDNA is good enough for one PCR reaction.

주문정보

CAT.No	품명	규격	비고
C4234565-R	Universal cDNA Reverse Transcribed by Random Hexamer: Human Normal Tissues	100 rxn