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cDNA - Human Tumor Cell Line: Hela

Species: Human
Applications: NGS, qPCR
BioChain's PCR Ready First Strand cDNAs are available from tumor cell lines. 1 µl cDNA is sufficient for each PCR reaction.

02)576-6303
info@kyongshin.co.kr

제품소개

First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 10 µg total RNA was primed by an oligo dT primer and reverse transcription (RT) using MMLV reverse transcriptase in 40 µl final volume. RT reaction was stopped by heating at 65°C for 10 minutes. The cDNA is delivered in 1x RT buffer (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT). 1 µl cDNA is sufficient for one PCR reaction. The 5′ end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs.

주문정보

CAT.No	품명	규격	비고
C1255811	cDNA - Human Tumor Cell Line: Hela	40 rxn