Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Direct lysis protocol omits conventional cell-pelleting and resuspension steps.
Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
Prepare transfection-grade plasmid DNA in only 15 minutes.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Direct lysis protocol omits conventional cell-pelleting and resuspension steps.
Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
Prepare transfection-grade plasmid DNA in only 15 minutes.
Product Description
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE – Express Plasmid Midiprep kit utilizes a patented alkaline lysis method for purifying up to 1.2 mg of transfection-grade plasmid DNA directly fromE. coliculture without conventional cell-pelleting and resuspension steps. The ZP Express Lysis Buffer is simply added directly to the culture and the plasmid DNA is purified following neutralization and removal of debris using the patented ZymoPURE binding system.
The plasmid DNA is rapidly bound onto a column with a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The ZymoPURE – Express Plasmid Midiprep kit’s fast and simple protocol enables the recovery of highly concentrated transfection-grade plasmid DNA in just 15 minutes. As an added convenience, the ZymoPURE – Express Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
Binding Capacity
1.2 mg
Culture Input
25 ml
Elution Volume
≥ 150 µl
Endotoxin Levels
≤1 EU/µg of Plasmid DNA
Equipment
Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge
Format
Spin-Column
Processing Time
15 min
Purity
Typical Abs260/280 ≥1.8 and Abs260/230≥ 2.0
Recommended Sample Volume
≤ 25 ml
Size Range
Up to 200 kb
Yield
Up to 1.2 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 25 ml of overnight culture grown in LB for high copy number plasmids are 100 – 200 µg.
Yes, the RNase A is fairly stable at room temperature but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
The vacuum pump should be a single or double-staged unit capable of producing at least 400 mm Hg pressure at the vacuum manifold. If less pressure is applied, centrifuge the column prior to washing to remove any residual lysate/buffer remaining in the matrix.
Yes, however, the pressure needs to be around 400 mm Hg for the liquid to pass through the columns quickly. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.
No, the ZymoPURE kits are only compatible with ZymoPURE Wash 2 and we cannot disclose a substitution due to the sensitive nature of the recipe. Additional ZymoPURE Wash 2 can be purchased separately.
The binding capacity of the column is up 1.2 mg of plasmid, however, typical plasmid yields from directly processing 25 ml of overnight culture for a high copy plasmid is 100 – 200 µg.
Yes, up to 50 ml of bacterial culture grown in LB can be pelleted, resuspended, and processed with the kit. Please see the appendix in the protocol for more information.
Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase the elution efficiency of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on the column up to 10 minutes prior to centrifugation.
주문정보
CAT.No
품명
규격
비고
D4213
ZymoPURE - Express Plasmid Midiprep Kit
25 preps
Format: Spin-Column, Vacuum Manifold, or Centrifugation Sample Volume: 25 ml Processing Time: 15 minutes Elution Volume: ≥ 200 μl DNA Yield: ≤ 1.2 mg DNA Size Limits: ≤ 25 kb
