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Insulin (human) ELISA kit

Insulin in a polypeptide composed of 51 amino acids divided into two chains (A and B) linked by disulfide bonds. Both chains are organized as alpha helix.
Insulin is produced by the beta cells of the pancreatic islets in form of a precursor (prepro-ins...

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(010-8832-6303)

제품소개

Territorial Availability: Available worldwide directly through Bertin or your local distributor

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

Insulin (human) EIA kit

Correlated keywords:

measures

enzyme

immunoassays

ELISA

diabetes

spi-bio

Product Overview:
Insulin in a polypeptide composed of 51 amino acids divided into two chains (A and B) linked by disulfide bonds. Both chains are organized as alpha helix.
Insulin is produced by the beta cells of the pancreatic islets in form of a precursor (prepro-insulin) and is modified to obtain the pro-insulin (peptide signal elimination) and finally insulin (peptide C elimination).
The main function of the insulin is the regulation of the glucose concentration in the organism. The dysregulation of the production of insulin induced many disorders including diabetes.

Size	96 wells
Shipping	wet ice
Specificity	Pig Insulin: 100% Human Pro-insulin: not detected Human C-Peptide: not detected
Application Media	Cell culture supernatant, plasma and serum\|No extraction required; inhibitor cocktail to prevent hemolysis consequences
Sample volume	5 µL
Tracer	AcetylCholinesterase AChE
Detection Limit	0.3 µIU/mL (11.5 pg/mL)
Standard Curve Range	0.4 µIU/mL - 52 µIU/mL (15.4 pg/mL – 2000 pg/mL)
Custom Code	3822000000
UNSPSC code	41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: INSULIN

Wilcox G. Insulin and Insulin Resistance. Clin Biochem Rev Vol 26 May 2005

PRODUCT EXPLANATION: KIT DEVELOPMENT

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

주문정보

CAT.No	품명	규격	비고
A05322	Insulin (human) ELISA kit	96 wells	AcetylCholinesterase AChE