Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
▶For complete, in vitro methylation of DNA for methylation analysis.
▶Methylation of chromatin DNA for DNA accessibility studies.
▶Inhibition of endonucleases with overlapping CpG sequence recognition and [3H]-labeling of DNA.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
DESCRIPTION
CpG Methylase completely methylates all cytosines in double-stranded, non-methylated, and hemimethylated DNA in a 5'...CpG...3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis. This product is supplied with 10X Reaction Buffer and S-adenosylmethionine cofactor.
Additional Information
Enzyme Commission Number (EC 2.1.1.37)
Assay Condition
CpG Methylase in 1X CpG Reaction Buffer w/ 600 µM SAM. Incubate reaction mixtures at 30C (Performing the Methylase reaction at 30°C rather than 37°C promotes optimal reaction kinetics).
Heat Inactivation
65°C for 20 minutes
Source
Recombinant methylase is isolated from E. coli expressing the methyltransferase gene from Spiroplasma sp. strain MQ1.
Storage
Store at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles of reagents. Prolonged storage is at ≤ -70°C.
Unit Definition
One unit (U) is the amount of enzyme required to protect 1µg of λ DNA from cleavage by BstUI restriction endonuclease in a total reaction volume of 20 µl for 1 hour at 37°C.
주문정보
CAT.No
품명
규격
비고
E2010
CpG Methylase (M.Sssl)
200 U
Enzyme Concentration: 4 U/ μl Storage: -20°C Inactivation: 65°C for 20 minutes Standard Reaction Time: 2 hours One unit (U) is defined as the amount of enzyme required to protect 1 μg of λ DNA against cleavage by BstUI restriction endonuclease in a total
E2011
CpG Methylase (M.Sssl)
400 U
Enzyme Concentration: 4 U/ μl Storage: -20°C Inactivation: 65°C for 20 minutes Standard Reaction Time: 2 hours One unit (U) is defined as the amount of enzyme required to protect 1 μg of λ DNA against cleavage by BstUI restriction endonuclease in a total
