경신과학

Zymo Research

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DNA Degradase

HIGHLIGHTS

▶Quick and simple procedure for completely degrading DNA into its individual nucleotide component for quantitative analysis (e.g., whole-genome methylation analysis by HPLC, TLC, etc.).

▶1 hour, single-enzyme digest vs. conventional 6 - 16-hour multi-step enzyme digestion protocols

서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

DESCRIPTION

DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

Assay Condition	DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥ 1 hour.
Concentration	10 U/µl
Enzyme Inactivation	70C for 20 minutes
Storage	Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
Unit Definition	One unit (U) is the amount of enzyme required to degrade 1 µg of λ

주문정보

CAT.No	품명	규격	비고
E2016	DNA Degradase™	500 U	Enzyme Concentration: 10 U/ μl Storage: -20°C Inactivation: 70°C for 20 minutes Standard Reaction Time: 1 hour One unit (U) is defined as the amount of enzyme required to degrade 1 μg of λ DNA in a total reaction volume of 25 μl for 1 hour at 37°C.
E2017	DNA Degradase™	2,000 U	Enzyme Concentration: 10 U/ μl Storage: -20°C Inactivation: 70°C for 20 minutes Standard Reaction Time: 1 hour One unit (U) is defined as the amount of enzyme required to degrade 1 μg of λ DNA in a total reaction volume of 25 μl for 1 hour at 37°C.