Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
HIGHLIGHTS
Enrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing.
Inactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA/RNA Shield.
Remove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS.
서한형 대리
경신과학(주)
영업부
H.P) 010-8832-6303
제품소개
HIGHLIGHTS
Enrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing.
Inactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA/RNA Shield.
Remove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS.
DESCRIPTION
The Zymo Environ Water RNA Kit simplifies COVID-19 wastewater surveillance by increasing viral enrichment, inactivating pathogens, and improving limits of detection. The use of Water Concentrating Buffer (WCB) promotes the pelleting of viral capsids and protein-associated RNA, increasing SARS-CoV-2 RNA yields by more than 8-fold. DNA/RNA Shield allows for safe handling as it has been validated for inactivation of pathogens including SARS-CoV-2. Finally, the workflow yields highly concentrated, PCR inhibitor-free RNA ready for downstream applications including RT-qPCR, RT-ddPCR, and NGS.
Equipment Needed
Microcentrifuge, vortex, large 50 mL conical tube centrifuge (optional), cell disrupter/pulverizer (optional)
Format
Bead beating, spin column.
RNA Purity
High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations.
RNA Storage
RNA is eluted with RNase-free water and can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage.
Sample Inactivation
DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents, and is ideal for safe sample storage and transport at ambient temperatures.
Sample Input
Water, wastewater, sludge, sewage, biofilms, etc.
Sample Size
Up to 5 mL
Yield
Up to 10 µg RNA can eluted into ≥ 6 µL RNase-free water allowing for a highly concentrated sample.
