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ZymoBIOMICS Spike-in Control I (High Microbial Load)
Highlights
Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
in situ Quality Control: ensures each sample is quantified accurately
Unique composition: comprised of two microbes alien to the human microbiome
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
  • in situ Quality Control: ensures each sample is quantified accurately
  • Unique composition: comprised of two microbes alien to the human microbiome

Product Description


ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.

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Technical Specifications

BiosafetyThis product is not biohazardous as microbes have been fully inactivated.
Impurity Level< 0.01% foreign microbial DNA
Reference GenomesComplete reference genomes and 16S rRNA genes
Sample Storage-80°C
Storage SolutionDNA/RNA Shield

Resources


Q1: When should I use the Spike-in control?

Studies where strict bioburden is required, such as food or water studies, it would be useful to use the Spike-in Control.

High biomass samples (feces, cell culture, etc.) should be used with Spike-In I.

Low Biomass samples (swabs, water filters, etc.) should be used with Spike-In II.

Q2: What sample types are compatible with the spike-in control?

All bacterial sample types are compatible with the spike-in control.

주문정보

CAT.No 품명 규격 비고
D6320 ZymoBIOMICS Spike-in Control I (High Microbial Load) 25 Preps
D6320-10 ZymoBIOMICS Spike-in Control I (High Microbial Load) 250 Preps