Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Sensitive and specific detection of 5-hydroxymethlycytosine (5-hmC) from a variety of samples
Ideal for global 5-hmC quantitation and high throughput screening
Streamlined workflow can be completed in 3 hrs
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Sensitive and specific detection of 5-hydroxymethlycytosine (5-hmC) from a variety of samples
Ideal for global 5-hmC quantitation and high throughput screening
Streamlined workflow can be completed in 3 hrs
Product Description
The Quest 5-hmC DNA ELISA Kit is both sensitive and specific and can be used to accurately detect 5-hmC DNA in a variety of samples. The kit is compatible with a wide range of input DNA including intact vertebrate, plant, and microbial genomic DNA, as well as enzyme-digested and mechanically sheared fragments. The Control DNA Set included with this kit has been calibrated to accurately quantify the percent 5-hmC in sample DNA by use of a standard curve. Also, the fast, streamlined workflow is ideal when analyzing/screening large numbers of samples.
Technical Specifications
Detection
≥ 0.02% 5-hmC per 100 ng DNA
Equipment
Incubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.
Input
This protocol is optimized for 100 ng of input DNA/well. Compatible with DNA in the range of 20-200 ng/well.
Sample Source
Purified genomic DNA in water, Tris-EDTA, or similar.
Shipping of components is on blue and dry ice. Upon arrival, make sure to store buffers at 4°C, Control DNA and Anti-5hmC antibody at -20°C, and the Anti-DNA antibody can be stored at -20°C for 1 week (For long-term storage keep at -80°C).
The DNA ELISA controls are genomic DNA. Control A is E. coli genomic DNA as it comprises 0% 5-hmC. The other controls derive from various mouse tissues and the %5-hmC is specific to each tissue. The levels of 5-hmC are were quantify by mass-spec.
1. Make sure the correct buffers were used to coat, block and wash the plate. 2. Double-check that HRP developer and the Anti-DNA HRP antibody were stored and prepared correctly.
1. Make sure that standard curve developed properly (R2 > 0.9). 2. Verify that all samples and controls are prepared correctly, mixed well before dilution and addition to the wells. 3. Ensure that anti-DNA HRP antibody was stored and prepared correctly before addition to the wells. 4. %5-hmC levels of your sample might be too low for the assay to detect (detection limit is 0.01% in 100 ng of denatured DNA).
