Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Highest Specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA.
Premium Purity: RNase-free, protease-free, and animal-free.
Thermostable: Able to withstand high temperatures without denaturation or loss in activity.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Highest Specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA.
Premium Purity: RNase-free, protease-free, and animal-free.
Thermostable: Able to withstand high temperatures without denaturation or loss in activity.
Product Description
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.
It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex.
This enzymatic function is reliant upon the presence of Mg2+ ions, with a recommended concentration of 5 mM for most applications.
PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases.
This depends on the application. DSN is a highly versatile enzyme, and the input amount can be adjusted to meet specific requirements. The amount of DSN and incubation times will vary based on the desired product. For example, a typical amount of DSN used for the normalization of cDNA libraries ranges from 0.25 to 1 U.
To protect ssDNA in a pool of dsDNA/ssDNA, we recommend titrating the enzyme amount to determine the optimal conditions for ssDNA protection.
DSN is susceptible to EDTA inactivation at concentrations of 5 mM and above. We recommend performing an inactivation step by adding 5 mM EDTA at the reaction temperature for 5 minutes following digestion.
DSN is thermally stable and retains activity when heated to 98°C for 10 minutes. We suggest stopping all reactions with DSN by incubating with 5 mM EDTA for 5 minutes at 60°C.
Yes, we can customize your order request to meet your specific requirements. For bulk ordering, please fill out the form and one of our specialists will be in touch shortly.
