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Quick-16S Plus NGS Library Prep Kit (V1-V3)
Highlights
The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
100% automation ready with only a single PCR step and without the need for normalization.
Real-time PCR enables absolute microbial copy number quantification.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • The most streamlined NGS kit with only 30 minutes of hands-on time for 96 samples.
  • 100% automation ready with only a single PCR step and without the need for normalization.
  • Real-time PCR enables absolute microbial copy number quantification.

Product Description

The Quick-16S Plus NGS Library Prep Kit (V1-V3) is the fastest and simplest NGS library prep targeting the V1-V3 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a single qPCR/PCR for combined targeted amplification and barcode addition using specially designed primers. After pooling by equal volume, a single clean-up of the final library is performed, rather than massive AMPure® bead-based clean-ups. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.

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Technical Specifications

16S V1-V3 Primer Sequences (adapters not included)27f (AGRGTTYGATYMTGGCTCAG, 20 bp) and 515r (TBACCGCGGCTGCTGGCAC, 19 bp).
Amplicon SizeThe final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~492 bp.
Barcode Sequences10 bp barcodes are available to download here or by visiting the Documentation section of this Product Page.
Index PrimersUnique dual index (barcodes) to label individual samples.
Required EquipmentMicrocentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
Sample InputPurified microbial DNA ≤100 ng, free of PCR inhibitors.
Sequencing PlatformIllumina MiSeq® or NextSeq 1000/2000® without the need to add custom sequencing primers. We recommend the MiSeq® Reagent Kit v3 (600-cycle) or NextSeq 1000/2000® Reagent Kit P1/P2 (600-cycle). For assistance with sample sheet setup, see Appendix F.

주문정보

CAT.No 품명 규격 비고
D6440-PS1 Quick-16S Plus NGS Library Prep Kit (V1-V3) with Primer Set 1 96 rxns