Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Bisulfite-Converted Universal Methylated Human DNA Standard
Highlights
DNA that has been enzymatically methylated at all cytosines within a CpG dinucleotide context and bisulfite-converted for direct use in downstream methylation applications.
Provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
DNA that has been enzymatically methylated at all cytosines within a CpG dinucleotide context and bisulfite-converted for direct use in downstream methylation applications.
Provided with a primer set to amplify a fragment of DNA after bisulfite conversion.
Product Description
The Bisulfite-Converted Universal Methylated Human DNA Standard is designed to be used as a control for DNA bisulfite conversion and downstream analyses including PCR, MSP, and other amplification-based assays. This DNA is identical to our Universal Methylated Human DNA Standard, but has been bisulfite-converted using the EZ DNA Methylation – Direct (D5020) kit. This product is provided as a 20 ng/µl solution and contains enough for 50 reactions. The primer set included with the standard has been designed and validated to amplify a segment of the bisulfite-converted DNA.
Technical Specifications
Concentration
20ng/µl
Sample Storage
Store DNA standard at ≤ -20°C for up to 12 months. Prolonged storage should be ≤ -70°C.
All standards are enzymatically methylated by CpG Methylase in vitro at all CpG dinucleotides. The methylated DNA is very highly methylated (> 95% of all CpG sites).
D5015 is fully methylated human DNA which has been bisulfite converted using the EZ DNA Methylation-Direct Kit. When run in parallel with experimental samples through library prep, PCR, etc., this standard can be used to determine if there are issues with sample quality or conversion efficiency that may be negatively impacting downstream applications.
This standard is included with primers targeting human MLH1 mismatch repair gene, and will amplify non-methylated, methylated, and mixed methylated templates. By amplifying bisulfite converted DNA with the included primers, researchers can test and optimize their bisulfite PCR conditions.
We recommend ZymoTaq DNA Polymerase, a hot start Taq polymerase specifically designed to amplify bisulfite converted DNA. Another hot start DNA polymerase of choice may also be used.
