Q1: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q2: Can I use other DNase I enzymes (or sets)? (e.g. Qiagen DNase I)?
The kit is optimized with Zymo Research's DNase, however DNase from other manufacturers can be used. Follow the respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post-extraction, then purify using the RNA Clean & Concentrator-5.
Q3: How to store DNase-I following resuspension?
Aliquot and store at – 20°C. Minimize multiple freeze thaw cycles. Lyophilized DNase I is stable at room temperature.
Q4: Is this kit suitable for very small numbers of cells?
Yes, the Quick-DNA/RNA MicroPrep Plus (#D7005) is capable of isolating from single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q5: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q6: Is it possible to extract proteins with the Quick-DNA/RNA kit?
Yes, proteins can be precipitated. Please refer to the protocol appendix.
Q7: I ran out of DNA/RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. DNA/RNA Wash Buffer is also sold separately.
Q8: Is the DNA and RNA suitable for Next-Gen sequencing or other sensitive downstream applications
Yes, both DNA and RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, qPCR, etc.
Q9: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q10: Can I freeze my samples in DNA/RNA Lysis Buffer for later processing?
Yes, freeze samples at -80°C after samples are lysed/homogenized in DNA/RNA Lysis Buffer. Bring the sample to room temperature prior to RNA purification.
Q11: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 1 volume of DNA/RNA Lysis Buffer (1:1) and mix well. Proceed with DNA Purification.
