Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
ZymoBIOMICS Microbial Community Standard II (Log Distribution)
Highlights
Log abundance distribution: assess detection limit over a broad range (102 to 108 cells)
Accurate composition: assess the accuracy of microbiome measurements
Microbiomics QC: ideal for quality control of microbiome measurements
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Log abundance distribution: assess detection limit over a broad range (102 to 108 cells)
Accurate composition: assess the accuracy of microbiome measurements
Microbiomics QC: ideal for quality control of microbiome measurements
Product Description
ZymoBIOMICS Microbial Community Standard II (Log Distribution) is a mock microbial community consisting of eight bacterial and two fungal strains. This microbial standard can be used to assess the performance of microbiomics workflows and can also be used as a positive control for routine sequencing. Cells of the ten microbes were mixed in a log-distributed abundance, which allows the user to easily assess the detection limit of their microbiomics workflow. 75 µl of the standard contains about ~100 cells of Staphylococcus aureus, the organism of lowest abundance. If needed, the standard can be spiked into a sample matrix (e.g. soil and blood) to mimic real samples of interest. Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 89.1%, Pseudomonas aeruginosa - 8.9%, Bacillus subtilis - 0.89%, Saccharomyces cerevisiae - 0.89%, Escherichia coli - 0.089%, Salmonella enterica - 0.089%, Lactobacillus fermentum - 0.0089%, Enterococcus faecalis - 0.00089%, Cryptococcus neoformans - 0.00089%, and Staphylococcus aureus - 0.000089%.
We recommend working backwards from the analysis. First optimize your library prep with the ZymoBIOMICS Microbial Community DNA Standard (D6305, D6311) to assess bias in PCR, sequencing, bioinformatics, etc. Once your results have low/no bias when compared to the theoretical composition, then use the whole cell ZymoBIOMICS Microbial Community Standard (D6300, D6310) to assess bias in lysis efficiency.
No, the Microbial Community Standard is designed to assess the efficiency of the lysis method and is intended to be run in parallel with your samples. If all the organisms can be detected at/near the theoretical abundance, you can be confident your extraction method is unbiased.
The chemistry of the Qiagen Kits (QIAamp Powerfecal, DNeasy Powersoil) and the storage solution the Microbial Community Standard is stored in (DNA/RNA Shield) are not completely compatible. Instead, less input volume should be used (25 ul).
This may indicate an issue with the lysis method, which can be biased toward gram negative bacteria. See the Optimized Lysis Protocols under Documents for a table of bead beating devices and protocols validated by Zymo Research.
Devices found to underrepresent tough-to-lyse organisms under all tested conditions:
