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Angiotensin I ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Correlated keywords:

angiotensin

Enzyme

immunoassays

ELISA

spi-bio

spibio

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
The Renin-Angiotensin system (RAS) is essential for the control of blood pressure and homeostasis. It plays an important role in cardiovascular and renal disorders. Among the different peptides resulting from the proteolytic processing of angiotensinogen (the only substrate of Renin), the octapeptide Angiotensin II (AII) is the major hormone involved in the pathophysiology of hypertensive diseases as it mediates vasoconstrictor action.
Angiotensin I is the precursor of Angiotensin II. It is a decapeptide without apparent physiologic effect. It is converted in Angiotensin II by the Angiotensin-converting enzyme (ACE).
The peptidic sequence for Angiotensin I is highly conserved across mammalian species, this is why our kit cross-reacts with all mammalian samples.

Size: 96 wells

Shipping: dry ice

Stability: Store at -20degrees;shelf life 2 years maximum after production

Specificity:

Angiotensin I 100 %

Angiotensinogen < 0.001%

Angiotensin II < 0.001%

Application Media: Plasma

Tracer: AcetylCholinesterase AChE

Detection Limit: 10 pg/mL

Standard Curve Range: 16-2000 pg/mL

Custom Code: 3822000000

UNSPSC code: 41116104

PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: ANGIOTENSIN I

Speth RC, Giese MJ , Update on the Renin-Angiotensin System. J Pharmacol Clin Toxicol 1(1): 1004 (2013)

Ghazi L, Drawz P., Advances in understanding the renin-angiotensin-aldosterone system (RAAS) in blood pressure control and recent pivotal trials of RAAS blockade in heart failure and diabetic nephropathy. F1000Res. 2017 Mar 21;6. pii: F1000 Faculty Rev-297

Vian J, Pereira C, Chavarria V et al, The renin-angiotensin system: a possible new target for depression. BMC Med. 2017 Aug 1;15(1):144

Pacurari, Kafoury R., Tchounwou P.B. and Ndebele K., The Renin-Angiotensin-Aldosterone System in Vascular Inflammation and Remodeling.International Journal of Inflammation, Volume 2014, Article ID 689360

Paulis L, Unger T. Novel therapeutic targets for hypertension. Nat Rev Cardiol. 2010 Aug;7(8):431-41

PRODUCT EXPLANATION: KIT DEVELOPMENT

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5): 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

주문정보

CAT.No	품명	규격	비고
A05882	Angiotensin I ELISA kit	96 wells	AChE kits