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IL-4 (pig) ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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(010-8832-6303)

제품소개

Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

Interleukin-4

B-cell stimulatory factor 1

B cell growth factor 1

Lymphocyte stimulatory factor

Correlated keywords:

ELISA

Interleukine-4

B-cell stimulatory factor 1

B cell growth factor 1

Lymphocyte stimulatory factor

anti-apoptosis

anti-inflammatory

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Discovered in 1992, Interleukin-4 (IL-4) is a monomeric glycosylated polypeptide with a molecular weight of 18-20 kDa. IL-4 structure contains four alpha-helices which are anti-parallel and two anti-parallel beta-sheets.
IL-4 is also known as B-cell stimulatory factor 1 or B-cell growth factor 1 and as Lymphocyte stimulatory factor. IL-4 is a key regulator in humoral and adaptive immunity. IL-4 plays an essential role by promoting Th2 cell differentiation while inhibiting Th1 cell differentiation. IL-4 is an anti-apoptosis factor for the immune and non-immune cells. Due to its main function, IL-4 is an anti-inflammatory cytokine in autoimmune diseases. IL-4 deregulation could be a factor in the development of some diseases like allergic diseases or in the treatment resistance of tumors

Size: 96 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6months maximum after production

Specificity:

refer to technical booklet

Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix

Sample volume: 100 µL

Tracer: horseradish peroxidase HRP

Detection Limit: ≤0,02 ng/mL

Standard Curve Range: 0,02-2,00 ng/mL

Custom Code: 3822000000

UNSPSC code: 41116104

1. Stephen T. Smiley, Michael J. Grusby, Interleukin 4, 1998, Encyclopedia of Immunology 1451–1453

2. Carr C, Aykent S, Kimack NM, Levine AD, Disulfide assignments in recombinant mouse and human interleukin 4, 1991, Biochemistry. 30 (6): 1515–23-3. Walter MR, Cook WJ, Zhao BG et al, Crystal structure of recombinant human interleukin-4, 1992, J. Biol. Chem. 267 (28): 20371–6

4. J. Zamorano, M.D. Rivas, M. Pérez-G, Interleukin-4: A multifunctional cytokine, 2003, Inmunología Vol. 22/Num 2/215-224

5. Feldmann M, Brennan FM, Maini RN, Role of cytokines in rheumatoid arthritis, 1996, Annu.Rev Immunol; 14: 397-440

6. Zamorano J, Kelly AE, Austrian J et al, Costimulation of resting B lymphocytes alters the IL-4-activated IRS2 signaling pathway in a STAT6 independent manner: implications for cell survival and proliferation, 2011 Cell Res. 11: 44-54

주문정보

CAT.No	품명	규격	비고
A05420	IL-4 (pig) ELISA kit	96 wells	HRP kits