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CXCL10 (pig) ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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(010-8832-6303)

제품소개

Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

IP-10

C-X-C motif chemokine ligand 10

C-X-C motif chemokine 10

Correlated keywords:

ELISA

Cancer

Inflammatory

Chemokines

Swine

Porc

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
CXCL10 is also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 with a molecular weight close to 10 kDa. CXCL10 is expressed by several cellular types like monocytes, endothelial cells and fibroblasts stimulated by the interferon-gamma (INF-γ). The receptor of this chimiokine is CXCR3 that is shared with CXCL9 and CXCL11. CXCL-10 plays an important role in the recruitment of the immune cells to sites of inflammation. It is included in physiological process like bone marrow colony formation and angiogenesis but also in some disorders like Th1-type human inflammatory diseases, or cancer.

Size: 96 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6months maximum after production

Specificity:

refer to technical booklet

Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix

Sample volume: 100 µL

Tracer: horseradish peroxidase HRP

Detection Limit: ≤0,08 ng/mL

Standard Curve Range: 0,08-10,00 ng/mL

Custom Code: 3822000000

UNSPSC code: 41116104

Luster A. D., Unkeless J. C., Ravetch J. V. Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. 1985 Nature 315:672.

Booth V,. Keizer D.W., Kamphuis M.B., et al. The CXCR3 binding chemokine IP-10/CXCL10: structure and receptor interactions. 2002 Biochemistry. 41 (33): 10418–25.

Dufour J. H., Dziejman M., Liu M. T., et al. IFN-γ-Inducible Protein 10 (IP-10; CXCL10)-Deficient Mice Reveal a Role for IP-10 in Effector T Cell Generation and Trafficking. 2002 J Immunol 168:3195-3204

Gottlieb, A. B., Luster A. D., Posnett D. N., et al. Detection of a γ-interferon-induced protein IP-10 in psoriatic plaques. 1988 J. Exp. Med. 168:941-948.

Angiolillo A.L., Sgadari C., Taub D.D., et al. Human interferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. 1995 The Journal of Experimental Medicine. 182 (1): 155–162.

주문정보

CAT.No	품명	규격	비고
A05419	CXCL10 (pig) ELISA kit	96 wells	HRP kits