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MIP-1β (pig) ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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제품소개

Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

MIP-1β

CCL4

Chemokine (C-C motif) ligand 4

Correlated keywords:

ELISA

Chemokine

CCL4

Chemokine (C-C motif) ligand 4

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Macrophage inflammation protein-1β (MIP-1β), also known as CCL4, is a C-C Chemokine produced by macrophages after stimulation by bacterial endotoxins. This chemokine is crucial for immune responses towards infection and inflammation. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells.
When produced by CD8+ T cells, MIP-1β is also a major HIV-suppressive factor

Size: 96 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6months maximum after production

Specificity:

refer to technical booklet

Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix

Sample volume: 100 µL

Tracer: horseradish peroxidase HRP

Detection Limit: ≤0,02 ng/mL

Standard Curve Range: 0,02-2,00 ng/mL

Custom Code: 3822000000

UNSPSC code: 41116104

1. Sherry B, Tekamp-Olson P, Gallegos C et al. Resolution of the two components of macrophage inflammatory protein 1, and cloning and characterization of one of those components, macrophage inflammatory protein 1 beta. December 1988 J. Exp. Med. 168 (6): 2251–9

2. Ren M, Guo Q, Guo L, et al. Polymerization of MIP-1 chemokine (CCL3 and CCL4) and clearance of MIP-1 by insulin-degrading enzyme.December 2010 EMBO J. 29 (23): 3952–66.

3. Bystry RS, Aluvihare V, Welch KA et al. B cells and professional APCs recruit regulatory T cells via CCL4. December 2001 Nat. Immunol. 2 (12): 1126–32

4. Cocchi F, DeVico AL, Garzino-Demo A et al. Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells. December 1995 Science. 270 (5243): 1811–5

주문정보

CAT.No	품명	규격	비고
A05417	MIP-1β (pig) ELISA kit	96 wells	HRP kits