Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Also known as type II interferons, IFN-gamma is a glycoprotein of 146 amino acids.
IFN-gamma is a cytokine critical for the innate and adaptive immunity. It is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response and by T helper cells and cytotoxic T lymphocyte (CTL) as part of the adaptive immunity.
IFN-gamma has antiviral, immunoregulatory, and anti-tumor properties.
IFN-gamma:
> promotes the NK cell activity,
> increases the antigen presentation by action on the lysosome activity of macrophages,
> promotes Th1 differentiation, and suppresses Th2
differentiation which would cause a humoral (antibody) response,
> modulates the production of IgG2a and IgG3 from activated plasma B cells,
> increases expression of class I MHC molecules as well as class II MHC on antigen-presenting cells,
> promotes adhesion and binding required for leukocyte migration.
IFN-gamma interacts with other cytokines, either in a synergistic (e.g., TNF) or antagonistic (e.g., IL-4) fashion.
Size: 96 wells
Shipping: wet ice
Stability: Store at +4degrees; shelf life 6 months maximum after production
Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix
Sample volume: 100 µL
Tracer: horseradish peroxidase HRP
Detection Limit: ≤0,08 ng/mL
Standard Curve Range: 0,08-10,00 ng/mL
Custom Code: 3822000000
UNSPSC code: 41116104
Vandenbroeck K., Dijkmans R., van Aerschot A. et al Engineering by PCR-based exon amplifi cation of the genomic porcine interferon-gamma DNA for expression in Escherichia coli. Biochem Biophys Res Commun. 1991 Nov 14;180(3):1408-15.
Billiau A. Interferon-gamma: biology and role in pathogenesis. Adv Immunol. 1996;62:61-130.
Paludan S.R. Interleukin-4 and interferon-gamma: the quintessence of a mutual antagonistic relationship.Scand J Immunol. 1998 Nov;48(5):459-68.
Boehm U., Klamp T., Groot M. et al. Cellular responses to interferon-gamma. Annu Rev Immunol. 1997;15:749-95
Schroder K., Hertzog P.J., Ravasi T. et al. Interferon-gamma: an overview of signals, mechanisms and functions.2004 J. Leukoc. Biol. 75 (2): 163–89.
