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E. coli Non-Methylated Genomic DNA
Highlights
Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions.


Product Description


E. coli non-methylated genomic DNA is from a Dam– and Dcm– strain (ER2925) of E. coli. It works perfectly as a negative control for DNA methylation analyses requiring DNA with absolutely no methylation.

Technical Specifications

Concentration250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Genotypeara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2
Storage≤ -20°C

Resources


Q1: How do you use these standards?

During NGS library prep, this standard can be spiked in to or run in parallel with experimental samples to monitor bisulfite conversion efficiency and/or workflow performance.

Q2: What reference genome should I use for alignment?

The reference genome of E. coli strain K-12 substrain MG1655 can be used for alignment and analysis. It can be accessed here.

주문정보

CAT.No 품명 규격 비고
D5016 E. coli Non-Methylated Genomic DNA 5 µg/20 µl