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BIOREAGENT(Bertin)
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Unacylated Ghrelin (mouse, rat) Easy Sampling ELISA kit
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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(010-8832-6303)

제품소개

Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor
Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview: 
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Bertin Bioreagent offers a totally new set of assays to measure Acylated & Unacylated Ghrelin, whatever the collection procedure.
Those assays are called Easy Sampling EIA kit because any blood sample collected can be used on any kind of protease inhibitors with or without acidification. From early discovery programs using our 384 wells plate format to select your GOAT inhibitor, to the pre-clinical or clinical stage, Bertin Bioreagent has a solution.


Size: 96 wells
Shipping: wet ice
Stability: Store at -20degrees; shelf life 2 years maximum after production
Application Media: Plasma, buffer|Blood collection on inhibitor then dilution prior assay
Tracer: AcetylCholinesterase AChE
Detection Limit: refer to technical booklet
Standard Curve Range: 2-250 pg/mL
Custom Code: 3822000000
UNSPSC code: 41116104


PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

. Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: GHRELIN

Kojima M, Kangawa K. Ghrelin: structure and function. Physiol. Rev ( 2005), 85:495-522

Müller TD, Nogueiras R, Andermann ML et al. Ghrelin. Mol Metab. 2015 Mar 21;4(6):437-60

Bluet-Pajot MT, Tolle V, Zizzari P, Tomasetto C, Grouselle D, Epelbaum J. Ghrelin: A striking example of neuroendocrine peptide pleiotropy. Med Sci (Paris), August 1, 2005, 21 (8-9): 715-21

de Faria Barros A et al. Is there association between acyl-ghrelin and inflammation in hemodialysis patients? J Bras Nefrol. (2013) 35(2):120-126

Grousselle D et al. Variations des peptides dérivés de la préproghréline au cours du repas dans l’anorexie mentale restrictive. Poster GIR-AFDAS-TCA2014

Delhanty P et al. Des-acyl ghrelin analogs prevent high-fat-diet-induced dysregulation of glucose homeostasis. FASEB J. (2013) 27(4):1690-1700

Costantini, V et al. GSK1614343, a Novel Ghrelin Receptor Antagonist, Produces an Unexpected Increase of Food Intake and Body Weight in Rodents and Dogs. Neuroendocrinology (2011);94:158–168

Porporato E, Filigheddu N et al. Acylated and unAcylated Ghrelin impair skeletal muscle atrophy in mice. J. Clinical Invest (2013) 123(2): 611-622

Sentissi O, Epelbaum J, Olié JP, Poirier MF. Leptin and Ghrelin Levels in Patients With Schizophrenia During Different Antipsychotics Treatment: A Review. Schizophrenia Bulletin (2008) 34(6), 1189–1199

PRODUCT EXPLANATION: KIT VALIDATION

Valentin MA, Ma S, Zhao A, Legay F, Avrameas A. Validation of immunoassay for protein biomarkers: Bioanalytical study plan implementation to support pre-clinical and clinical studies. J Pharm Biomed Anal. (2011) 55(5) : 869-877

European Medicines Agency. Guideline on bioanalytical method validation, 21 July 2011

CITATIONS OF GHRELIN KITS

Hassouna, Grouselle D, Chiappetta G et al. Combination of Selective Immunoassays and Mass Spectrometry to Characterize Preproghrelin-Derived Peptides in Mouse Tissues. Front Neurosci. 2017 Apr 20;11:211

Beauloye V, Diene G, Kuppens R et al. High unacylated ghrelin levels support the concept of anorexia in infants with prader-willi syndrome. Orphanet J Rare Dis. 2016 May 4;11(1):56

Erden I, Uçak H, Demir B et al. Serum ghrelin levels in patients with Behcet's disease.Postepy Dermatol Alergol. 2016 Dec;33(6):450-456

Gagnon J, Anini Y. Insulin and Norepinephrine Regulate Ghrelin Secretion from a Rat Primary Stomach Cell Culture. Endocrinology (2012) doi 10.1210/en.2012-1040

Lin Li R, Sherbet D et al. Profound Hypoglycemia in Starved, Ghrelin-deficient Mice Is Caused by Decreased Gluconeogenesis and Reversed by Lactate or Fatty Acids. JBC (2012)

Lu X et al. Postprandial inhibition of gastric ghrelin secretion by long-chain fatty acid through GPR120 in isolated gastric ghrelin cells and mice. Am J Physiol Gastrointest Liver Physiol (2012)doi:10.1152/ajpgi.00541.2011

Robert F et al. Targeting Protein Synthesis in a Myc/mTOR-Driven Model of Anorexia-Cachexia Syndrome Delays Its Onset and Prolongs Survival. Cancer Res (2012) doi:10.1158/0008-5472.CAN-11-2739

Teubner B et al. Inhibition of ghrelin O-acyltransferase attenuates food deprivation-induced increases in ingestive behavior. Hormones and Behavior (2013)

주문정보

CAT.No 품명 규격 비고
A05318 Unacylated Ghrelin (mouse, rat) Easy Sampling ELISA kit 96 wells AChE kits