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Streptavidin-AChE Tracer
Acetylcholinesterase (AChE®) is extracted from the electric organ of the electric eel, Electrophorus electricus. 100 determinations (100 dtn) correspond to 10 ml of a solution of Streptavidin-AChE Tracer at 1 UE/mL
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010-8832-6303

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Territorial Availability: Available worldwide directly through Bertin Bioreagent or your local distributor
Technical Warning: UltraPure Water (Milli-Q or equivalent) is needed for this tracer
Product Overview: 
Acetylcholinesterase (AChE®) is extracted from the electric organ of the electric eel, Electrophorus electricus. 100 determinations (100 dtn) correspond to 10 ml of a solution of Streptavidin-AChE Tracer at 1 UE/mL

Size: 100 dtn
Shipping: wet ice
Stability: Store at -20degrees; shelf life 1 year maximum after production. Once reconstituted, store at +4degrees for one month maximum.
Application(s):

EIA

ELISA

Suggested assay protocol:

- Reconstitute the vial with 10 mL of Buffer of your choice : Triton® X-100 EIA Buffer, standard EIA Buffer WARNING : use UltraPure water free of any organic contaminant. - Rinse the plate three times - Distribute the Streptavidin-AChE Tracer, the volume distributed is equal to the volume of your first reaction step - Example: If the volume of your first reaction is 100 µL, distribute 100 µL of this Tracer - Incubate 1h30 at room temperature under agitation at 300 rpm on an orbital shaker - Rinse the plate three times - Distribute 200 µL of Ellman’s reagent - Incubate the plate at room temperature under agitation at 300 rpm on an orbital shaker - Measure absorbance at 405 nm or at 414 nm each 30 minutes until the desired absorbance

Specificity:
Tracer: AcetylCholinesterase AChE
Formulation: lyophilized
Custom Code: 3822000000

주문정보

CAT.No 품명 규격 비고
A04750 Streptavidin-AChE Tracer 100 dtn AChE kit components