Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
HIGHLIGHTS
Highest Activity: Complete and efficient DNA digestion for sensitive downstream applications like RNA sequencing.
RNase-free and Protease-free: Rigorously tested and guaranteed to be free of contaminating RNases and proteases.
Exceptional Stability: Maintains activity after vigorous vortexing and multiple freeze-thaw cycles.
DESCRIPTION
Zymo Research DNase I is a recombinant endonuclease that cleaves single- and double-stranded DNA. It digests DNA more efficiently than other DNase I, making it optimal for sensitive applications like NGS (RNA-seq) and PCR. It is extensively purified, and quality controlled to ensure RNase-free and protease-free.
Zymo Research DNase I is lyophilized and supplied with DNA digestion buffer. It dissolves in water within seconds and remains stable for up to 10 freeze-thaw cycles. Customized, OEM, and bulk orders are available.
Unit Definition
One Kunitz causes an increase in absorbance at 260 nm of 0.001 per minute per ml, at 25°C, pH 7.5, when acting on salmon sperm DNA according to the assay method of Kunitz. One Unit of DNase I is equivalent to 12 Kunitz.
Storage
After reconstitution, place on ice until ready to use or store frozen aliquots (-20°C).
Reconstitution
Add DNase/RNase-free water to the lyophilized DNase I, mix by gentle inversion. Avoid phosphate buffer and calcium chelators.
Inactivation
Heat inactivate at 75 °C for 10 min with 5 mM EDTA.
Remove contaminating DNA from RNA or protein samples
Clean up of RNA samples prior to RT-PCR
DNA-free RNA sample preparation prior to RNA-seq
Remove DNA templates after in vitro transcription
DNA labeling by nick-translation
Reduce viscosity in cell culture or cell lysate
DNase I footprinting
주문정보
CAT.No
품명
규격
비고
E1010
DNase I Set
250 U
Provided lyophilized (250 U) with DNA Digestion Buffer (4 ml)
