Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Simple: Fast spin-column procedure yields pure yeast genomic DNA without using glass beads or phenol.
Versatile: Efficient DNA isolation from a broad spectrum of fungal species susceptible to Zymolyase.
High-Quality: Isolated genomic DNA is ready for Southern blotting, PCR, restriction enzyme digestions, etc.
Product Description
The YeaStar Genomic DNA Kit is designed for reliable and efficient isolation of genomic DNA from a broad spectrum of fungal species, including Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The kit is based on highly efficient enzyme lysis and Zymo-Spin column technology. Each standard prep yields about 7 - 20 µg of DNA with a size distribution of 35 - 60 kb. The resulting genomic DNA can be used for direct analysis including Southern blotting, PCR, restriction endonuclease digestion, etc.
Technical Specifications
Applicable For
Isolated DNA is well suited for downstream applications such as Southern blotting, PCR, restriction endonuclease digestion, Next-Generation Sequencing and other sensitive applications.
Elution Volume
≥ 60 µl TE or DNase-free Water
Equipment
Microcentrifuge, heat block/bath
Processing Time
1.5 hours
Processing Volume
≤ 1.5 ml of Culture (1-5 x 107 cells)
Purity
Typical A260/A280 & A260/A230 ≥ 1.8
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Size Range
Capable of recovering 35-60 kb sized fragments of genomic and mitochondrial DNA.
Protocol I and II are almost same, except that chloroform is used in Protocol I. Protocol I usually gives more recovery of DNA by 30-80% compared to Protocol II. Protocol II is chloroform-free. Chloroform is not provided.
Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya, Kloekera, Candida, Kluyveromyces, Debaryomyces, Lipomyces, Eremothecium, Metschikowia, Endomyces, Pichia, Hansenula, Pullularia, Hanseniaspora, Saccharomyces, Saccaromycodes, Saccharomycopsis, Schizosaccahromyces, Torulopsis
Zymolyase is very efficient, but here are some tips to maximize DNA yield. 1. Use fresh and early log phase cells, which are more susceptible to the lytic properties of Zymolyase. 2. Using less cells as input can improve overall lysis efficiency. 3. Zymolyase digestion time can also be increased to 2 – 16 hours.
