Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
For isolation of plasmid DNA for downstream applications such as PCR, transformation, hybridization, etc.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Simple: Quickly and easily rescue plasmid from yeast.
Efficient Isolation: Works well with low-copy and hard to isolate plasmids.
High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Product Description
The Zymoprep Yeast Plasmid Miniprep I is a simple and efficient yeast plasmid miniprep that is based on the E. coli alkaline lysis method but using Zymolyase as the first solution. The kit provides all the necessary reagents for plasmid isolation from S. cerevisiae, C. albicans and S. pombe, and any fungi whose cell walls are susceptible to yeast lytic enzyme lysis. The procedure is simple and efficient, and there is no need for glass beads or phenol. Reliably recover plasmid DNA from yeast colonies, patches on plates, or as liquid cultures. The system is ideal for low-copy number and hard to isolate plasmids. Eluted plasmid DNA can be used directly for E. Coli transformation, PCR, and Southern blot analysis.
Technical Specifications
Applicable For
Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications
Elution Volume
≥ 35 µl
Equipment
Microcentrifuge & Heat Block/Bath
Processing Volume
≤ 1.5 ml of Culture
Sample Source
Cell Culture (Colonies/Patches or Liquid Culture)
Size Range
No Size Limit
Yield
Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures. In order to generate more plasmid, the plasmid is typically transformed into E. Coli , cultured, and isolated using a traditional E. Coli plasmid prep.
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya, Kloekera, Candida, Kluyveromyces, Debaryomyces, Lipomyces, Eremothecium, Metschikowia, Endomyces, Pichia, Hansenula, Pullularia, Hanseniaspora, Saccharomyces, Saccaromycodes, Saccharomycopsis, Schizosaccahromyces, Torulopsis
