Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
High-quality cell free RNA is easily and robustly purified from up to 1 ml of plasma, serum, or other biological fluids
Immediately ready for downstream applications including RT-qPCR and Next-Generation Sequencing
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
HIGHLIGHTS
Quick & Easy: Simple spin-column based isolation from up to 3 mL of plasma, serum, or other biological liquids. No phenol/chloroform or precipitation.
Highest Yields: Efficient isolation of total cell-free RNA. Recover up to 515 times more microRNA.
Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, nCounter, etc.
DESCRIPTION
The Quick-cfRNA Serum & Plasma Kit enables simple, reliable, and efficient preparation of high-quality circulating cell-free RNA (including protein-bound, exosomal, miRNA and other small RNAs) from serum, plasma and other biological fluids. The kit does not use toxic phenol-chloroform or cumbersome protein precipitation and removal steps. Recovered cell-free RNA scales linearly relative to the sample input volume up to 3 ml. ZymoSpin™ Technology enables isolation of ultra-pure cell-free RNA suitable for all subsequent analyses, such as RT-qPCR and NGS. Empower your discovery of circulating RNA biomarkers.
Elution Volume
cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
Processing Time
Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours.
Purity
High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
Required Equipment
Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
Sample Type
Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
Size
RNA ≥ 17 nt.
Yield
Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA.
Q1: Can saliva be used as sample input?
Saliva must be cleared to remove debris/mucus (12,000xg for 15 min at room temperature). Up to 1 ml cleared salvia can be used per extraction.
Proportional scale up relative to input volume (from 0.2 ml to 1 ml; 55y male plasma). Triplicates of purified samples used to generate RT-qPCR (Bioline; EPIK miRNA Select Kit, hsa-miR-16-5p assay).
2x and 8x more yields achieved from low and high input volumes, respectively, compared to the next best product from other supplier. Triplicates of purified samples from different kits used to generate RT-qPCR data (Bioline; EPIK miRNA Select Kit, hsa-miR-16-5p Assay). Kit from competitor Q cannot process input volume higher than 0.2 ml. Common sample source used (55y male plasma).
