Increased Viral RNA Yields for the Earliest Detection of COVID-19 Outbreaks

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Safe, Affordable SARS-CoV-2 Surveillance

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8x More SARS-CoV-2 RNA

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Zymo Environ Water RNA Kit

Get 8x More SARS-CoV-2 from Wastewater for the Earliest Detection of COVID-19

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The Zymo Environ Water RNA Kit includes Water Concentrating Buffer (WCB). 5ml of SARS-CoV-2 positive wastewater was processed with and without WCB (n=2). RT-qPCR showed the addition of WCB shifted average Ct values earlier by ~3 (an 8-fold increase in viral recovery).

The Most Effective Wastewater Workflow Ever

Enhanced viral enrichment; get 8x more viral RNA
Pathogen inactivation for safe handling
PCR inhibitors removed in one spin
≥ 6 µl elution improves limits of detection

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PCR Inhibitor-Free RNA

bar graph comparing HeLa Spike-in (positive control) versus HeLa Spike-in (wastewater)

Extracted Wastewater RNA is free of PCR Inhibitors. 50K HeLa cells were spiked into DNA/RNA Shield (positive extraction control) and into wastewater. RNA was extracted (n=2) and RT-qPCR (Quasar 670) analysis showed the ΔCt is <0.5, indicating the extraction system effectively removes inhibitors in wastewater.

Download the Success Story

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Learn everything about how Los Angeles County Sanitation District successfully tracks COVID-19 surges in wastewater.

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Water Concentrating Buffer Inactivates COVID-19

The Water Concentrating Buffer included in the Zymo Environ Water RNA Kit inactivates SARS-CoV-2 for safe sample processing, while preserving the integrity of viral RNA.

Viral inactivation demonstrated by plaque formation assay. Vero-E6 cells were infected with serial dilutions of SARS-CoV-2, strain Muc-IMB-1. Virus was applied without pre-treatment, or incubated for 48 hours with agarose overlay before fixation with formaldehyde. Plaques were visualized with crystal violet staining.

What People Are Saying

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"Have been working on wastewater SARS-Co-V-2 surveillance for over 9 months now. Tried several methods and kits for concentration of virus particles in wastewater. This kit from Zymo was one of the top performers. What makes it unique is the water concentrating buffer. Will strongly recommend this product."

-Shireen K.

University of Virginia

FAQ

Yes, the purification steps can be performed on a vacuum manifold. It is recommended that the wash steps and elution steps be performed with a microcentrifuge.

Yes. Filters can be cut or left intact and inserted into the ZR BashingBead™ Lysis Tube. Add 750 µl DNA/RNA Shield and proceed with step 3 of Sample Homogenization.

Wastewater contains solids including cellulose. These solids can bind up viral particles and RNA. Mechanical disruption of solids increases RNA yields dramatically.

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